Rubin Lab Methods Book
- Quarantine Stocks
- Fly Food Recipe
- Population Cages
- Some Modified Drosophila Transformation Vectors
- Modified Carnegie Vectors
- Modified Neomycin Selection Vectors
- Vectors Permitting Promotion Fusions With Chloramphenicol Acetyltransferase Transcriptional Model 13
- Sevenless Expression Casettes
- Pcawc Vector
- Hss Derivatives
- G418 Selection
- Drosophila Tissue Culture
- Cytological Examination Of Polytene Chromosomes
- Chromosome In Situ Hybridization Using Biotin Labeled Probes
- In Situ Hybridization To Embryo Sections Using 35s Riboprobes
- Whole Mount In Situ Hybridization With A Nonradioactive Probe
- Fixation And Pretreatment For Imaginal Disc Whole Mount Nonradioactive In Situ Hybridization 50
- Immunostaining Drosophila Embryos
- Staining Eye Imaginal Discs
- A. Labelling With Antibodies
- B. Cobalt Sulphide Staining
- C. Basic Fuchsin Staining
- Antibody And Cobalt Staining Of Pupal Eye Discs
- β-Galactosidase Detection In Embryos
- β-Galactosidase Detection In Imaginal Discs
- Adult Head Frozen Tissue Sections
- β-Galactosidase Activity Staining
- Antibody Staining
- Whole Mount Staining Of Drosophila Retina For βGalactosidase Activity
- Ems Mutagenesis
- Local Mutagenesis By Imprecise P-Element Excision
- X Ray Mutagenesis
- Microscopic Analysis Of The Adult Retina
- Somatic Mosaic Analysis In The Eye
- Preparation Of Adult Drosophila For Scanning Electron Microscopy
- Photomicrography
- Genomic DNA Preps
- Rna Isolation Using Guanidine Hydrochloride
- Plasmid Rescue From P[Lacz] Lines
- Preparation Of Probes For Footprinting Experiments
- Purine Cleavage Sequence Markers
- Dnaasei Footprinting
- Primer Labelling For Primer Extension Assay
- Primer Extension
- Oligo-Labelled Dna Probes
- Northern Blots Using Riboprobes
- Polyclonal And Monoclonal Antibodies
- Polyclonal Antibody
- Monoclonal Antibody
- Alkali Plasmid Preps
- Small-Scale Plasmid Dna Preparation
- Maxi Plasmid Preps
- Growth Of M13 Phage
- Gel Purification Of Oligonucleotides
- Purification Of Oligonucleotides By Thin Layer Chromatography (Tlc)
- Procedure For Site-Directed Mutagenesis
- Site-Directed Mutagenesis
- Double Stranded Sequencing
- Preparation And Purification Of E. Coli β-Galactosidase Fusion Proteins And Their Use As Immunogens
- Fusion Proteins For Antibody Production
- Making Trp E Fusion Proteins For Antibody Production
- Gst Fusions For Antibody Production
- Solid Phase Radioimmune Assay For Igg
- Protein Gels - Sds Polyacrylamide
- Western Blots
- Electroelution Of Proteins Using Isco Apparatus
- Making Footprinting Extracts From Drosophila Heads
- High Efficiency Electro-Transformation Of E.Coli
- Bromodeoxyuridine (Brdu Or Budr) Incorporation And Visualization
- Lambdasorb Liquid Phage Prep
- Small-Scale Preparation Of Bacteriophage Lambda Dna
- Preparation Of Single Stranded Dna (For In Vitro Mutagenesis)
- Site Directed Mutagenesis
- Bacteriophage Lambda Library Screening: Summary
- Rapid Small Scale Isolation Of Drosophila Dna And Rna
- Cat Assay: Drosophila Transformants
- Chromosome Walking Using Cosmids
- Deep Pseudopupil In White- And Red-Eyed Flies
- Making Seminar Slides 192
- Appendix I: Reagents