Rubin Lab Methods Book

  1. Quarantine Stocks
  2. Fly Food Recipe
  3. Population Cages
  4. Some Modified Drosophila Transformation Vectors
  5. Modified Carnegie Vectors
  6. Modified Neomycin Selection Vectors
  7. Vectors Permitting Promotion Fusions With Chloramphenicol Acetyltransferase Transcriptional Model 13
  8. Sevenless Expression Casettes
  9. Pcawc Vector
  10. Hss Derivatives
  11. G418 Selection
  12. Drosophila Tissue Culture
  13. Cytological Examination Of Polytene Chromosomes
  14. Chromosome In Situ Hybridization Using Biotin Labeled Probes
  15. In Situ Hybridization To Embryo Sections Using 35s Riboprobes
  16. Whole Mount In Situ Hybridization With A Nonradioactive Probe
  17. Fixation And Pretreatment For Imaginal Disc Whole Mount Nonradioactive In Situ Hybridization 50
  18. Immunostaining Drosophila Embryos
  19. Staining Eye Imaginal Discs
  20. A. Labelling With Antibodies
  21. B. Cobalt Sulphide Staining
  22. C. Basic Fuchsin Staining
  23. Antibody And Cobalt Staining Of Pupal Eye Discs
  24. β-Galactosidase Detection In Embryos
  25. β-Galactosidase Detection In Imaginal Discs
  26. Adult Head Frozen Tissue Sections
  27. β-Galactosidase Activity Staining
  28. Antibody Staining
  29. Whole Mount Staining Of Drosophila Retina For βGalactosidase Activity
  30. Ems Mutagenesis
  31. Local Mutagenesis By Imprecise P-Element Excision
  32. X Ray Mutagenesis
  33. Microscopic Analysis Of The Adult Retina
  34. Somatic Mosaic Analysis In The Eye
  35. Preparation Of Adult Drosophila For Scanning Electron Microscopy
  36. Photomicrography
  37. Genomic DNA Preps
  38. Rna Isolation Using Guanidine Hydrochloride
  39. Plasmid Rescue From P[Lacz] Lines
  40. Preparation Of Probes For Footprinting Experiments
  41. Purine Cleavage Sequence Markers
  42. Dnaasei Footprinting
  43. Primer Labelling For Primer Extension Assay
  44. Primer Extension
  45. Oligo-Labelled Dna Probes
  46. Northern Blots Using Riboprobes
  47. Polyclonal And Monoclonal Antibodies
  48. Polyclonal Antibody
  49. Monoclonal Antibody
  50. Alkali Plasmid Preps
  51. Small-Scale Plasmid Dna Preparation
  52. Maxi Plasmid Preps
  53. Growth Of M13 Phage
  54. Gel Purification Of Oligonucleotides
  55. Purification Of Oligonucleotides By Thin Layer Chromatography (Tlc)
  56. Procedure For Site-Directed Mutagenesis
  57. Site-Directed Mutagenesis
  58. Double Stranded Sequencing
  59. Preparation And Purification Of E. Coli β-Galactosidase Fusion Proteins And Their Use As Immunogens
  60. Fusion Proteins For Antibody Production
  61. Making Trp E Fusion Proteins For Antibody Production
  62. Gst Fusions For Antibody Production
  63. Solid Phase Radioimmune Assay For Igg
  64. Protein Gels - Sds Polyacrylamide
  65. Western Blots
  66. Electroelution Of Proteins Using Isco Apparatus
  67. Making Footprinting Extracts From Drosophila Heads
  68. High Efficiency Electro-Transformation Of E.Coli
  69. Bromodeoxyuridine (Brdu Or Budr) Incorporation And Visualization
  70. Lambdasorb Liquid Phage Prep
  71. Small-Scale Preparation Of Bacteriophage Lambda Dna
  72. Preparation Of Single Stranded Dna (For In Vitro Mutagenesis)
  73. Site Directed Mutagenesis
  74. Bacteriophage Lambda Library Screening: Summary
  75. Rapid Small Scale Isolation Of Drosophila Dna And Rna
  76. Cat Assay: Drosophila Transformants
  77. Chromosome Walking Using Cosmids
  78. Deep Pseudopupil In White- And Red-Eyed Flies
  79. Making Seminar Slides 192
  80. Appendix I: Reagents