MM mm
P[ry+] / P[ry+] ; ry / ry x CyO / Sp ; Sb, «2-3 / TM6,
Ubx
2nd generation (here occurs the excision):
M (single males) mm
P[ry+] / CyO ; Sb, «2-3 / ry x CyO / Sco ; ry / ry
3rd generation:
M (single males, ry eye color) mm
E{P} / CyO ; ry / ry x CyO / Sco ; ry /ry
4th generation (brother-sister cross):
MM mm
E{P} / CyO ; ry / ry x E{P} / CyO ; ry / ry
established stock, if excision is lethal; check homozygous flies for visible phenotypes.
III. Chromosome P Insertions
1st generation:
MM mm
P[ry+] , ry / P[ry+] , ry x Ki, pp, «2-3 / Ki, pp, «2-3
2nd generation (here occurs the excision):
M (single males) mm
P[ry+] , ry / Ki, pp, «2-3 x TM3, ry / rf10 (ftz, e, ry)
3rd generation:
M (single males, ry eye color) mm
E{P}, ry / TM3, ry x TM3, ry / rf10
4th generation (brother-sister cross):
MM mm
E{P}, ry / TM3, ry x E{P}, ry / TM3, ry
established stock, if lethal; check for visible mutations in homozygous flies
X Chromosome P Insertions:
1st generation:
mm MM
P[ry+] / P[ry+] ; ; ry / ry x + / -- ; ; Ki, pp, «2-3/ Ki, pp, «2-3
2nd generation (here occurs the excision):
M (single males) mm
P[ry+] / -- ; ; Ki, pp, «2-3 / ry x FM6 / dnd ; ; ry /
ry
3rd generation:
m (single virgins, ry eye color) MM
E{P} / FM6 ; ; ry / ry x FM6 / -- ; ; ry / ry
established balanced stock, if excision is lethal; check for visible phenotypes in
homozygous flies.