Biohazard Flow Cytometry

The Biohazard Flow Cytometry Core provides high-speed cell sorting and multiparameter flow cytometric analysis for work involving agents posing a documented or potential biohazard. The laboratory is set up to enable high-speed sorting and analysis of unfixed human primary cells and cell lines under conditions that comply with safety standards recommended by the International Society for Analytical Cytology and approved by our Institutional Biosafety Committee. Resources are also available for sorting and analysis of various infectious agents, including many human pathogens such as HIV requiring up to Biosafety Level 2 containment.

Services

Access the Services

  • High-speed cell sorting using MoFlo-XDP or FACSAria
  • Multiparameter flow cytometric analysis using MoFlo-XDP, FACSAria, FACSCanto II and FACSCalibur
  • Laser scanning cytometry using Compucyte iCys
  • Magnetic antibody-bead-based sorting using Miltenyi Biotec SuperMACS
  • Consultation on biocontainment requirements for cell sorting experiments
  • Assistance with biohazard flow cytometry experimental design and data analysis
  • Training on operation of high-speed cell sorters and analyzers under biocontainment conditions
Related Shared Facilities
Related Shared Facilities

Analytical Imaging

Center Affiliations
Center Affiliations

Einstein-Montefiore Center For AIDS Research (CFAR)

Selected Publications

AIDS Research and Therapy, 2008 ;5:3 Journal of Virology, 2008 Aug;82(15):7591-600 Journal of Virology, 2008 Jun;82(11):5562-72 Journal of Virology, 2008 Mar;82(6):3078-89 PLoS Pathogens, 2008 Feb 8;4(2):e28

Location and Contacts

Biohazard Flow Cytometry Chanin 308/309 (main lab), 624C; Price/Block 159