• Overview
• Lab Members
• Movies
  • Yeast Movies
  • Dictyostelium movies
  • Tumor Cell (MTLn3) Movies
• Movies from Paper
  • Bailly et al, 2000 Molecular Biology of the Cell
  • Kedrin et al., 2008 Nature Methods
  • Kempiak et al. 2003 Journal of Cell Biology
  • Sahai et al 2005 BMC Biotechnology
  • Wyckoff et al. 2000 Cancer Research
  • Xue et al. 2006 Cancer Research
• Helpful ImageJ
  • Generating time lapse movies from time lapse z series
  • Eliminating x-y drift in time lapse movies
  • Marking areas of interest in time lapse movies

Jeffrey E. Segall | Ramon Cabrera | Serena Chiang | Sangeeta Jayakar | Lizandra Jimenez 

Education:

Post-graduate Training:

Professional Employment:

Professional Society Membership:

Awards and Honors:

Publications

A. Original Communications in Reviewed Journals

1. Chiang, P.K., Guranowski, A. and J.E. Segall (1981) Irreversible Inhibition of S-Adenosylhomocysteine Hydrolase by Nucleoside Analogs. Arch. Bioc. Bioph. 207:175:184.
2. Segall, J.E., Manson, M.D. and H.C. Berg. (1982) Signal Processing Times in Bacterial Chemotaxis. Nature 296:855-857.
3. Block, S.M., Segall, J.E. and H.C. Berg. (1982) Impulse Responses in Bacterial Chemotaxis. Cell 31:215-226.
4. Segall, J.E., Ishihara, A. and H.C. Berg. (1985) Chemotactic Signaling in Filamentous Cells of Escherichia coli. J. Bacter. 161:51-59.
5. Segall, J.E., Block, S.M, and H.C. Berg. (1986) Temporal Comparisons in Bacterial Chemotaxis. Proc. Natl. Acad. Sci. USA 83:8987-8991.
6. Segall, J.E., Fisher, P.R. and G. Gerisch. (1987) Selection of Chemotaxis Mutants of Dictyostelium discoideum. J. Cell Biol. 104:151-161.
7. Segall, J.E.. (1988) Analysis of Responses of Dictyostelium discoideum Amoebae to Chemoattractant Concentration Changes. J. Muscle Res. Cell Mot. 9:481-490.
8. Segall, J.E., Bominaar, A., Wallraff, E. and R. de Wit. (1988) Analysis of a Dictyostelium Chemotaxis Mutant with Altered Chemoattractant Binding. J. Cell Sci. 91:479-489.
9. Andre, E., Brink, M., Gerisch, G., Isenberg, G., Noegel, A., Schleicher, M., Segall, J. E., and E. A. Wallraff. (1989) A Dictyostelium Mutant Deficient in Severin, an F-actin Fragmenting Protein, Shows Normal Motility and Chemotaxis. J. Cell Biol. 108:985-995.
10. Brinke, J., Gerisch, G., Isenberg, G., Noegel, A.A., Segall, J.E., Wallraff, E., and M. Schleicher. (1990) A Dictyostelium Mutant lacking an F-actin Crosslinking Protein, the 120 kD Gelation Factor. J. Cell Biol. 111:1477-1489.
11. Barisic, K., Mollner, S., Noegel, A. A., Gerisch, G., and J. E. Segall. (1991) cDNA Sequence of Cyclophilin from Dictyostelium discoideum. Developmental Genetics, 12:50-53.
12. Segall, J.E. (1992) Behavioral responses of streamer F mutants of Dictyostelium discoideum: effects of cyclic GMP on cell motility. J. Cell Sci., 101:589-597. Segall, J.E. and G. Gerisch. (1989) Genetic Approaches to Cytoskeleton Function and the Control of Cell Motility. Ann. Rev. in Cell Biology: Cytoplasm and Cell Motility. 1:44-50.
13. Segall, J.E. (1993) Polarization of yeast cells in spatial gradients of mating factor. Proc. Natl. Acad. Sci. 90:8332-8336.
14. Segall, J.E., Kuspa, A., Shaulsky, G., Ecke, M., Maeda, M., Gaskins, C., Firtel, R.A., and W.G. Loomis. (1995) A MAP kinase necessary for receptor-mediated activation of adenylate cyclase in Dictyostelium. J. Cell Biol. 128:405-413.
15. Segall, J.E., Tyrech, S., Boselli, L., W., Masseling, S., Chan, A., Helft, J, Jones, J. and J. Condeelis. (1996) EGF stimulates lamellipod extension and chemotaxis in metastatic mammary adenocarcinoma cells by an actin-dependent mechanism. Clin. Exp. Met. 14:61-72.
16. Gaskins, C., Clark, A.M., Aubry, L., Segall, J.E., Firtel, R.A. (1996) The Dictyostelium MAP kinase ERK2 regulates multiple, independent developmental pathways. Genes and Dev. 10:118-128.
17. Knetsch, M.L.W., Epskamp, S.J.P., Schenk, P.W., Segall, J.E., and B.E. Snaar-Jagalska. (1996) Both G-protein and ras-dependent activation of extracellular signal regulated kinase 2 is antagonized by intracellular cAMP in Dictyostelium discoideum. EMBO J. 15:3361-3368.
18. Leberer, E., Wu, C., Leeuw, T., Fourest-Lieuvin, A., Segall, J.E., and D. Y. Thomas (1996) Functional characterization of the Cdc42p binding domain of yeast Ste20p protein kinase. EMBO J. 16:83-97.
19. Edmonds, B.T., Wyckoff, J., Yeung, Y.-G., Wang, Y., Stanley, E.R., Jones, J., Segall, J.E., and J. Condeelis (1996) Elongation factor-1alpha is an overexpressed actin binding protein in metastatic rat mammary adenocarcinoma. J. Cell Science 109:2705-2714.
20. Chan, A., Raft, S., Bailly, M., Wyckoff, J., Segall, J. and Condeelis, J. (1998) EGF stimulates an increase in actin nucleation and filament number at the leading edge of the lamellipod in mammary adenocarcinoma cells. J. Cell Sci. 111:199-211.
21. Wang, Y., Liu, J. and J.E. Segall (1998) MAP kinase function in amoeboid chemotaxis. J. Cell Sci. 111:373-383.
22. Wang, Y. and J.E. Segall (1998) The Dictyostelium MAP kinase DdERK2 functions as a cytosolic protein in complexes with its substrates in chemotactic signal transduction. Biochem. Biophys. Res. Comm. 244:149-155.
23. Bailly, M., Yan, L., Whitesides, G.M., Condeelis, J.S., and J.E. Segall (1998) Regulation of protrusion shape and adhesion to the substratum during chemotactic responses of mammalian carcinoma cells. Exp. Cell Res. 241:285-299.
24. Farina, K., Wyckoff, J., Rivera, J., Lee, H., Segall, J., Condeelis, J. and Jones, J. (1998) Cell motility of tumor cells visualized in living intact primary tumors using green fluorescent protein. Cancer Res. 58:2528-2532.
25. Wyckoff, J.B., Insel, L., Khazaie, K., Lichtner, R.B., Condeelis, J.S. and J.E. Segall (1998) Suppression of ruffling by EGF in chemotactic cells. Exp. Cell Res. 242:100-109.
26. Bailly, M., Macaluso, F., Cammer, M., Chan, A., Segall, J.E., and J.S. Condeelis. (1999) Relationship between Arp2/3 complex and the barbed ends of actin filaments at the leading edge of carcinoma cells after EGF stimulation. J. Cell Biol. 145:331-345.
27. Hill, K., Welti, S., Yu, J., Murray, J.T., Yip, S., Condeelis, J.S., Segall, J.E., Backer, J.M. (2000) Specific requirement for the p85-p110alpha PI 3-kinase during EGF-stimulated actin nucleation in breast cancer cells. J. Biol. Chem. 275:3741-3744.
28. Chan,A.Y., Bailly,M., Zebda,N., Segall,J.E, and J.S. Condeelis (2000) Role of cofilin in epidermal growth factor-stimulated actin polymerization and lamellipod protrusion. J Cell Biol. 148:531-542.
29. Wyckoff, J.B.,Jones, J.G., Condeelis, J. C., and J E. Segall (2000) A critical step in metastasis: In vivo analysis of intravasation at the primary tumor. Cancer Res. 60:2504-2511.
30. Wyckoff, J.B., Segall, J.E. and Condeelis, J.C. (2000) The collection of the motile population of cells from a living tumor. Cancer Res. 60:5401-5404.Bailly,M. , Wyckoff,J.B., Bouzahzah, B., Hammerman,R., Sylvestre,V., Cammer,M., Pestell,R.G., and J. E. Segall (2000) Distribution of the EGF receptor in chemotaxing adenocarcinoma cells. Mol. Biol. Cell. 11:3873-3883. Hill, K.M., Huang, Y, Yip, S.-C., Yu, J., Segall, J.E., and J.M. Backer. (2001) N-terminal domains of the class IA phosphoinositide 3-kinase regulatory subunit play a role in cytoskeletal but not mitogenic signaling. J. Biol. Chem. 276:16374-16378.
31. Bailly, M., Ichetovkin, I., Grant, W., Zebda, N., Machesky, L.M., Segall, J.E., and J. Condeelis (2001) The F-actin side binding activity of the Arp 2/3 complex is essential for actin nucleation and lamellipod extension. Curr. Biol. 11: 620-625.
32. Bouzahzah, B., Albanese, C., Ahmed, F., Pixley, F., Lisanti, M. P, Segall, J.E., Condeelis, J., Joyce, J., Minden, A, Der, C.J., Chan, A., Symons, M. and R. G. Pestell. (2001) Rho Family GTPases Regulate Mammary Epithelium Cell Growth and Metastasis Through Distinguishable Pathways. Mol. Med. 7: 816–830.
33. Wang, W., Wyckoff, J.B., Frohlich, V.C., Oleynikov, Y., Huttelmaier, S., Zavadil, J., Cermak, L., Bottinger, E.P., Singer, R.H., White, J.G., Segall, J.E., and J.S. Condeelis. (2002) Single cell behavior in metastatic primary mammary tumors correlated with gene expression patterns revealed by molecular profiling. Cancer Res. 62:6278-88.
34. Vallier, L.G., Segall, J.E., and M. Snyder. (2002) The alpha-factor receptor C-terminus is important for mating projection formation and orientation in Saccharomyces cerevisiae. Cell Motil Cytoskeleton 53:251-66.
35. Butty, A.C., Perrinjaquet, N., Petit, A., Jaquenoud, M., Segall, J.E., Hofmann, K., Zwahlen, C., and M. Peter. (2002) A positive feedback loop stabilizes the guanine-nucleotide exchange factor Cdc24 at sites of polarization. EMBO J 21:1565-76.
36. Ahmed F, Wyckoff J, Lin EY, Wang W, Wang Y, Hennighausen L, Miyazaki J, Jones J, Pollard JW, Condeelis JS, Segall JE. (2002) GFP Expression in the Mammary Gland for Imaging of Mammary Tumor Cells in Transgenic Mice. Cancer Res. 62:7166-9.
37. Wang W, Wyckoff JB, Wang Y, Bottinger EP, Segall JE, Condeelis JS. (2003) Gene expression analysis on small numbers of invasive cells collected by chemotaxis from primary mammary tumors of the mouse. BMC Biotechnol. 3:13-25.
38. Kempiak, S., Yip, J.-L., Backer, J., and J. E. Segall (2003) Local signaling by the EGF receptor. J. Cell Biol.162:781-787.
39. Wyckoff J, Wang W, Lin EY, Wang Y, Pixley F, Stanley ER, Graf T, Pollard JW, Segall J, Condeelis J (2004 ) A paracrine loop between tumor cells and macrophages is required for tumor cell migration in mammary tumors. Cancer Res. 64:7022-9.
40. Wang W, Goswami S, Lapidus K, Wells AL, Wyckoff JB, Sahai E, Singer RH, Segall JE, Condeelis JS. (2004) Identification and testing of a gene expression signature of invasive carcinoma cells within primary mammary tumors. Cancer Res. 64:8585-94.
41. Kempiak SJ, Yamaguchi H, Sarmiento C, Sidani M, Ghosh M, Eddy RJ, Desmarais V, Way M, Condeelis JS, Segall JE (2005) J. Biol. Chem. 280:5836-5842.
42. Goswami S, Sahai E, Wyckoff J, Cox D, Pixley F, Stanley ER, Segall JE, Condeelis, J (2005) Macrophages promote the invasion of carcinoma cells in vitro via a paracrine loop. Cancer Res. 65:5278-83.
43. Yamaguchi H, Lorenz M, Kempiak S, Sarmiento C, Coniglio S, Symons M, Segall J, Eddy R, Miki H, Takenawa T, Condeelis J. (2005) Molecular mechanisms of invadopodium formation: the role of the N-WASP-Arp2/3 complex pathway and cofilin. J Cell Biol. 168(3):441-52.
44. Sahai E, Wyckoff J, Philippar U, Segall JE, Gertler F, Condeelis J. (2005) Simultaneous imaging of GFP, CFP and collagen in tumors in vivo using multiphoton microscopy. BMC Biotechnol. 5:14-22.
45. Soon L, Mouneimne G, Segall J, Wyckoff J, Condeelis J. (2005) Description and characterization of a chamber for viewing and quantifying cancer cell chemotaxis. Cell Motil Cytoskeleton. 62(1):27-34.
46. Xue C, Wyckoff J, Liang F,Sidani M, Violini S, Tsai K-L, Zhang Z-Y, Sahai E, Condeelis J, Segall JE (2006) EGF receptor overexpression results in increased tumor cell motility in vivo coordinately with enhanced intravasation and metastasis. Cancer Res. 66:192-7
47. Xue C, Liang F, Mahmood R, Vuolo M, Wyckoff J, Qian H, TsaiK-L, Kim M, Locker J, Zhang Z-Y, Segall JE (2006 ) ErbB3-dependent motility and intravasation in breast cancer metastasis. Cancer Res. 66: 1418-1426.
48. Chen S, Segall JE (2006) EppA, a putative substrate of DdERK2, regulates cyclic AMP relay and chemotaxis in Dictyostelium discoideum. Eukaryot Cell. 5:1136-46.
49. Wyckoff JB, Wang Y, Lin EY, Li JF, Goswami S, Stanley ER, Segall JE, Pollard JW, Condeelis J. (2007) Direct visualization of macrophage-assisted tumor cell intravasation in mammary tumors. Cancer Res. 67:2649-56.
50. Wang W, Wyckoff JB, Goswami S, Wang Y, Sidani M, Segall JE, Condeelis JS. (2007) Coordinated regulation of pathways for enhanced cell motility and chemotaxis is conserved in rat and mouse mammary tumors. Cancer Res. 67:3505-11.
51. Pu J, McCaig CD, Cao L, Zhao Z, Segall JE, Zhao M. (2007) EGF receptor signalling is essential for electric-field-directed migration of breast cancer cells. J Cell Sci. 120 :3395-403.
52. Kedrin D, Gligorijevic B, Wyckoff J, Verkhusha VV, Condeelis J, Segall JE, van Rheenen J (2008) Intravital imaging of metastasis through an orthotopic Mammary Imaging Window. Nature Methods. Nature Methods. 5:1019-21
53. Kedrin D, Wyckoff JB, Hynes NE, Arteaga CL, Segall JE (2009) ERBB1 and ERBB2 have distinct functions in tumor cell invasion and intravasation. Clin. Cancer Res. 15: 3733-9.
54. Hernandez L, Smirnova T, Kedrin D, Wyckoff J, Stanley ER, Cox D, Muller WJ, Pollard JW, van Rooijen N, Segall JE (in press) EGF/CSF-1 Induced Breast Cancer Cell Invasion is Triggered by Heregulin Beta 1 and CXCL12. Cancer Res.
55. Gligorijevic B, Kedrin D, Segall JE, Condeelis J, van Rheenen J. (2009) Dendra2 photoswitching through the Mammary Imaging Window. J Vis Exp. 28:1278.
56. Pang H, Flinn R, Patsialou A, Wyckoff J, Roussos ET, Wu H, Pozzuto M, Goswami S, Condeelis JS, Bresnick AR, Segall JE, Backer JM. (2009) Differential enhancement of breast cancer cell motility and metastasis by helical and kinase domain mutations of class IA phosphoinositide 3-kinase. Cancer Res. 69(23):8868-76.
57. Piatkevich KD, Hulit J, Subach OM, Wu B, Abdulla A, Segall JE, Verkhusha VV. (2010) Monomeric red fluorescent proteins with a large Stokes shift. Proc Natl Acad Sci U S A. 2010 Mar 23;107(12):5369-74.
58. Hernandez L, Magalhaes MA, Coniglio SJ, Condeelis JS, Segall JE: Opposing roles of CXCR4 and CXCR7 in breast cancer metastasis. Breast cancer research : BCR 2011, 13(6):R128.
59. Medina-Ramirez CM, Goswami S, Smirnova T, Bamira D, Benson B, Ferrick N, Segall J, Pollard JW, Kitsis RN: Apoptosis inhibitor ARC promotes breast tumorigenesis, metastasis, and chemoresistance. Cancer research 2011, 71(24):7705-7715.
60. Loudig O, Brandwein-Gensler M, Kim RS, Lin J, Isayeva T, Liu C, Segall JE, Kenny PA, Prystowsky MB: Illumina whole-genome complementary DNA-mediated annealing, selection, extension and ligation platform: assessing its performance in formalin-fixed, paraffin-embedded samples and identifying invasion pattern-related genes in oral squamous cell carcinoma. Human pathology 2011, 42(12):1911-1922.
61. Boimel PJ, Cruz C, Segall JE: A functional in vivo screen for regulators of tumor progression identifies HOXB2 as a regulator of tumor growth in breast cancer. Genomics 2011, 98(3):164-172.
62. Roussos ET, Balsamo M, Alford SK, Wyckoff JB, Gligorijevic B, Wang Y, Pozzuto M, Stobezki R, Goswami S, Segall JE et al: Mena invasive (MenaINV) promotes multicellular streaming motility and transendothelial migration in a mouse model of breast cancer. Journal of cell science 2011, 124(Pt 13):2120-2131.
63. Smirnova T, Adomako A, Locker J, Van Rooijen N, Prystowsky MB, Segall JE: In vivo invasion of head and neck squamous cell carcinoma cells does not require macrophages. The American journal of pathology 2011, 178(6):2857-2865.
64. Henkels KM, Farkaly T, Mahankali M, Segall JE, Gomez-Cambronero J: Cell invasion of highly metastatic MTLn3 cancer cells is dependent on phospholipase D2 (PLD2) and Janus kinase 3 (JAK3). Journal of molecular biology 2011, 408(5):850-862.
65. Boimel PJ, Smirnova T, Zhou ZN, Wyckoff J, Park HI, Coniglio SJ, Patel P, Qian BZ, Stanley ER, Bresnick AR et al: Contribution of CXCL12 secretion to invasion of breast cancer cells. Breast cancer research : BCR 2012, 14(1):R23.
66. Coniglio SJ, Eugenin E, Dobrenis K, Stanley ER, West BL, Symons MH, Segall JE: Microglial stimulation of glioblastoma invasion involves EGFR and CSF-1R signaling. Molecular medicine (Cambridge, Mass) 2012.
67. Harris T, Jimenez L, Kawachi N, Fan JB, Chen J, Belbin T, Ramnauth A, Loudig O, Keller CE, Smith R et al: Low-Level Expression of miR-375 Correlates with Poor Outcome and Metastasis While Altering the Invasive Properties of Head and Neck Squamous Cell Carcinomas. The American journal of pathology 2012, 180(3):917-928.
68. Smirnova T, Zhou ZN, Flinn RJ, Wyckoff J, Boimel PJ, Pozzuto M, Coniglio SJ, Backer JM, Bresnick AR, Condeelis JS et al: Phosphoinositide 3-kinase signaling is critical for ErbB3-driven breast cancer cell motility and metastasis. Oncogene 2012, 31(6):706-71.
69. Kim RS, Avivar-Valderas A, Estrada Y, Bragado P, Sosa MS, Aguirre-Ghiso JA, Segall JE. (2012). Dormancy signatures and metastasis in estrogen receptor positive and negative breast cancer. PloS one. 7(4):e35569. PMID: 22530051 / PMCID: PMC3329481.
70. Ishihara D, Dovas A, Hernandez L, Pozzuto M, Wyckoff J, Segall JE, Condeelis JS, Bresnick AR, Cox D. (2013) Wiskott-Aldrich syndrome protein regulates leukocyte-dependent breast cancer metastasis. Cell Rep. 4(3):429-36. PubMed PMID: 23911287.
71. Zhou ZN, Sharma V, Beaty B, Roh-Johnson M, van Rooijen N, Kenny P, Wiley S, Condeelis J, Segall JE. (2013) Autocrine HBEGF expression promotes breast cancer intravasation, metastasis and macrophage-independent invasion in vivo. Oncogene 33(29):3784-93.
72. Ishihara D, Dovas A, Hernandez L, Pozzuto M, Wyckoff J, Segall JE, Condeelis JS, Bresnick AR, Cox D. WASp is required for tumor associated macrophages to promote breast carcinoma cell invasion and metastasis. Cell Reports. 2013 4:429-36.
73. Kim S, Yao J, Suyama K, Qian X, Qian BZ, Bandyopadhyay S, Loudig O, De Leon-Rodriguez C, Zhou ZN, Segall J, Macian F, Norton L, Hazan RB. Slug promotes survival during metastasis through suppression of Puma-mediated apoptosis. Cancer Res. 2014 Jul 15;74(14):3695-706.
74. Qian X, Hulit J, Suyama K, Eugenin EA, Belbin TJ, Loudig O, Smirnova T, Zhou ZN, Segall J, Locker J, Phillips GR, Norton L, Hazan RB. p21CIP1 mediates reciprocal switching between proliferation and invasion during metastasis. Oncogene. 2013 May 2;32(18):2292-303.

B. Books, Chapters in Books, and Review Articles

1. Segall, J.E. and G. Gerisch (1989) Genetic approaches to cytoskeleton function and the control of cell motility. Curr. Op. Cell Biol.: Cytoplasm and Cell Motility. 1:44-50.
2. Gerisch, G., Segall, J.E. and E. Wallraff. (1989) Isolation and Behavioral Analysis of Mutants Defective in Cytoskeletal Proteins. Cell Motility and the Cytoskeleton. 14:75-79.
3. Segall, J. E. (1990) Mutational Studies of Amoeboid Chemotaxis using Dictyostelium discoideum. In: Biology of the Chemotactic Response, (J. Armitage and J. Lackie, eds.) Cambridge University Press, Cambridge, pp 241--272.
4. Jones, J., Segall, J.E. and J. Condeelis. (1991) Molecular analysis of amoeboid chemotaxids: parallel observation in amoeboid phagocytes and metastatic tumor cells. In Cytokines and Cell Motility (I. Goldberg and E. Rosen, eds.) Birkhauser Verlag, New York.
5. Manson, M.D., and J.E. Segall (1992) Chemotaxis. In The Encyclopedia of Microbiology (J. Lederberg, ed.) Academic Press, San Diego.
6. Condeelis, J., Jones, J., and J. E. Segall (1992) Chemotaxis of metastatic tumor cells: clues to mechanisms from the Dictyostelium paradigm. Cancer and Metastasis Reviews 11:55-68.
7. Mann, S. P. Devreotes, S. Eliott, K. Jermyn, A. Kuspa, M. Fechheimer, R. Furukawa, C. Parent, J. Segall, G. Shaulsky, P. Vardy, J. Williams, and R. Firtel. (1994) Cell biological, molecular genetic, and biochemical methods to examine Dictyostelium. In: "Cell Biology: A Laboratory Handbook" (ed J. Celis), Academic Press 1:412-451.
8. Cammer, M., Wyckoff, J. and J.E. Segall (1997) Computer-assisted analysis of single cell motility. In: Basic Cell Culture Protocols (eds. J.M. Walker and J.W. Pollard), Methods in Molecular Biology, 75:459-470.
9. Zigmond, S.H., Foxman, E.F. and J.E. Segall (1998) Chemotaxis Assays for Eukaryotic Cells. In: Current Protocols in Cell Biology (eds) Bonifacino, J.S., et al. New York, John Wiley & Sons. (in press).
10. Bailly, M. Condeelis, J. and Segall, J.E. (1998) Chemoattractant-induced lamellipod extension. Microscopy Research and Technique. 43:433-443
11. Segall, J.E. (1999) Cell Polarization: Chemotaxis gets cracking. Current Biology 9:R46-R48.
12. Pestell, R.G., Albanese, C., Reutens , A.T., Segall, J.E., Lee, R.J., and Arnold, A. (1999) The cyclins and cyclin-dependent kinase inhibitors in hormonal regulation of proliferation and differentiation. Endocr Rev 20:501-34.
13. Condeelis, J.S., Wyckoff, J., and J. E. Segall (2000) Imaging of cancer invasion and metastasis using green fluorescent protein. Eur. J. Cancer 36:1671-1680.
14. Condeelis. J.S., Wyckoff, J.B., Bailly, M., Pestell, R., Lawrence,D., Backer, J., and Jeffrey E. Segall (2001) Lamellipodia in invasion. Sem. Canc. Biol. 11:119-128.
15. Condeelis, J. and J.E. Segall (2003) Intravital imaging of cell movements in tumors. Nat. Rev. Canc. 3:921-930.
16. Condeelis J, Song X, Backer J, Wyckoff J and JE Segall (2003) Chemotaxis of cancer cells during invasion and metastasis. In: Cell Motility: from molecules to organisms. (Editors: Anne Ridley, Michelle Peckham, Peter Clark) Wiley & Sons. 175-188.
17. Kempiak, S.J. and J.E. Segall (2004) Stimulation of cells using EGF-coated magnetic beads. Sci STKE. 218:pl1.
18. Meili, R., Richard A. Firtel, R.A. and J. E. Segall. (2004) Molecular Mechanisms of Chemotaxis in Amoebae. In: Chemotaxis (editor Michael Eisenbach) U.K., Imperial College Press. 253-294.
19. Bozzaro, S., Fisher P.R., Loomis, W., Satir, P. and J. E. Segall (2004) Guenther Gerisch and Dictyostelium, the microbial model for ameboid motility and multicellular morphogenesis. Trends Cell Biol. 14:585-9.
20. Wyckoff J, Segall JE, and J Condeelis (2005) “Chapter 22: Single cell imaging in animal tumors in vivo” in Live Cell Imaging: a Laboratory Manual, Eds.: D.L. Spector and R.D. Goldman. CSHL. Cold Spring Harbor, NY. Pp. 409 - 422.
21. Wang W, Goswami S, Sahai E, Wyckoff JB, Segall JE, Condeelis JS. (2005) Tumor cells caught in the act of invading: their strategy for enhanced cell motility. Trends Cell Biol. 15:138-45.
22. Ingman, W., Wyckoff J, Xue C, Lin EY, Wang W, Goswami S, Pollard JW, Condeelis J, and JE Segall (2005) Imaging Invasion and Metastasis In vivo. In: Cell Motility in Cancer Invasion and Metastasis (editor Alan Wells), Kluwer Academic Publishers.
23. Condeelis J, Singer R, Segall JE (2005) The great escape: When cancer cells hijack the genes for chemotaxis and motility. Ann. Rev. Cell Dev. Biol. 21: 695-718.
24. Sidani M, Wyckoff J, Xue C, Segall JE, Condeelis J. (2006) Probing the microenvironment of mammary tumors using multiphoton microscopy. J Mammary Gland Biol Neoplasia. 11:151-63.
25. Wyckoff JB, Condeelis J, Segall JE (2006) Growth factor receptors – doing more than just stimulating tumor growth? BIF Futura. 21:219-223.
26. Kedrin D, Wyckoff J, Sahai E, Condeelis J, Segall JE (2007) Imaging of tumor cell movement in vivo. Current Protocols in Cell Biology. 19.7.1 – 19.7.17.
27. Kedrin D, van Rheenen J, Hernandez L, Condeelis J, Segall JE (2007) Cell motility and cytoskeletal regulation in invasion and metastasis. J. Mam. Gland Biol. Neoplasia. 12: 143-52.
28. Hernandez L, Smirnova T, Wyckoff J, Condeelis J, Segall JE (2009) In vivo assay for tumor cell invasion. Methods in Molecular Biology. 571:227-38.
29. Zhou ZN, Boimel PJ, Segall JE: Tumor-stroma: In vivo assays and intravital imaging to study cell migration and metastasis. Drug discovery today Disease models 2011, 8(2-3):95-112.
30. Wyckoff J, Gligorijevic B, Entenberg D, Segall J, Condeelis J: The in vivo invasion assay: preparation and handling of collection needles. Cold Spring Harbor protocols 2011, 2011(10):1232-1234.
31. Wyckoff J, Gligorijevic B, Entenberg D, Segall J, Condeelis J: High-resolution multiphoton imaging of tumors in vivo. Cold Spring Harbor protocols 2011, 2011(10):1167-1184.
32. Locker J, Segall JE: Breast cancer: the matrix is the message. The American journal of pathology 2011, 178(3):966-968.
33. Childs G, Segall JE. (2012). Twists and turns of invasion. Nature cell biology. 14(4):337-9. PMID: 22469829.
34. Friedl P, Locker J, Sahai E, Segall JE. (2012) Classifying collective cancer cell invasion. Nat Cell Biol. 14(8):777-83.
35. Hulit J, Kedrin D, Gligorijevic B, Entenberg D, Wyckoff J, Condeelis J, Segall JE. (2012). The use of fluorescent proteins for intravital imaging of tumor cell invasion. In: In vivo cellular imaging using fluorescent proteins: methods and protocols. Hoffman, Robert M. (Ed.) Methods in Molecular Biology, Vol. 872. Humana Press, pp 15 – 30.
36. Entenberg D, Kedrin D, Wyckoff J, Sahai E, Condeelis J, Segall JE. (2013) Imaging tumor cell movement in vivo. Curr Protoc Cell Biol. Chapter 19:Unit19.7.
37. Coniglio S, Segall JE. Molecular mechanism of microglia stimulated glioblastoma invasion. Matrix Biology. 2013 32:372-80.

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