The CVEKL LAB

Ales Cvekl

ALES CVEKL, Ph.D.

Ph.D. in Biochemistry (1987). TheInstitute of Organic Chemistry and Biochemistry of the Czechoslovak Academy of Sciences, Prague, Czech Republic.

 

 

BIBLIOGRAPHY

A.        Original Communications in Reviewed Journals:

1.         Kodicek, M., A. Cvekl and Z. Vodrazka. 1981. Fluorescence study of the molecular conformation of human haemopexin. Int. J. Biol. Macromol. 3:397-398.

2.       Cvekl, A., K. Horska and K. Sebesta. 1988. On the mechanism of rifampicin inhibition of DNA dependent RNA polymerase from Escherichia coli. Collect. Czechoslovak Chem. Commun. 53:181-185.

3.        Cvekl, A., K. Horska, K. Sebesta, I. Rosenberg and A. Holy. 1988. The effect of divalent cations and core polymerase in abortive initiation by Escherichia coli DNA dependent RNA polymerase using phosphonate dinucleotide primers. Biocatalysis 1:275-281.

4.        Cvekl, A.,  K. Horska, K. Sebesta,  I. Rosenberg and A. Holy. 1989. Phosphonate analogues of dinucleotides as substrates for DNA dependent RNA polymerase in primed abortive initiation reaction. Int. J. Biol. Macromol. 11:33-38.

5.        Cvekl, A., K. Horska, K. Sebesta, I. Rosenberg and A. Holy. 1989. Trinucleotide AUA and UAU formation catalyzed by wheat germ RNA polymerase  II on poly(dA-dT) template.  Collect. Czechoslovak. Chem. Commun. 54:811-818.

6.         Schnapp, A., J. Clos, W. Haedelt, R. Schreck, A. Cvekl and I. Grummt. 1990. TIF-IB, a factor that confers promoter specificity to mouse RNA polymerase I. Nucleic Acids Res. 18:1385-1393.

7.         Horska, K.,  A. Cvekl,  I. Rosenberg,  A. Holy and K. Sebesta. 1990. DNA-dependent RNA polymerase mediated formation of methylphosphonyl internucleotide linkage. Coll. Czechoslovak Chem. Commun. 55:2769-2780.

8.        Cvekl, A. and K. Horska. 1990. Cibacron Blue inhibition of prokaryotic and eukaryotic DNA-dependent RNA polymerases. Collect. Czechoslovak Chem. Commun. 55:2769-2780.

9.        Cvekl, A., K. Horska, C. Vlcek and V. Paces. 1991. Protein binding AT-rich motifs flank the duckbA-globin enhancer. Gene (Amst.) 103:253-257.

10.      Cvekl, A. and V. Paces. 1992. Interactions between proteins bound to the duckbA-globin promoter and enhancer detected by the DNaseI footprinting. Gene (Amst.) 110: 225-228.

11.       Kantorow, M., A. Cvekl, C.M. Sax and J. Piatigorsky. 1993. Protein-DNA interactions of the mouse aA-crystallin control regions. Differences between expressing and non-expressing cells. J. Mol. Biol. 230:425-435.

12.       Klement, J.F., A. Cvekl* and J. Piatigorsky. 1993. Functional elements DE2A, DE2B and DE1A and the TATA box are required for activity of the chicken aA-crystallin gene in transfected lens epithelial cells. J. Biol. Chem. 268:6777-6784. (* Equal contribution of the first and second author).

13.       Tomarev, S.I., M. Duncan, H.J. Roth, A. Cvekl and J. Piatigorsky. 1994. Convergent evolution of crystallin gene regulation in squid and chicken: The AP-1/ARE connection. J. Mol. Evol. 39:134-143.

14.       Sax, C.M., A. Cvekl, M. Kantorow, B. Sommer, A.B. Chepelinsky and J. Piatigorsky. 1994. Identification of negative-acting and protein-binding elements in the mouse aA-crystallin -1556/-1165 region. Gene (Amst.) 144:163-169.

15.      Cvekl, A., C.M. Sax, E. H. Bresnick and J. Piatigorsky. 1994. Complex array of positive and negative elements regulates the chickenaA-crystallin gene: Involvement of Pax-6, USF, CREB and/or CREM and AP-1 proteins. Mol. Cell. Biol. 14:7363-7376.

16.      Cvekl, A., F. Kashanchi, C.M. Sax, J.N. Brady and J. Piatigorsky. 1995. Transcriptional regulation of the mouseaA-crystallin gene: Activation dependent on a cyclic AMP responsive element (DE1/CRE) and a Pax-6 binding site. Mol. Cell. Biol.15:653-660.

17.       Matlova, R., K. Horska, V. Benes, A. Cvekl and J. Sponar. 1995. Protein induced bending or flexing at the 5’-end of the duck bA-globin gene promoter. Gene (Amst.) 156:277-281.

18.      Cvekl, A., J.B. McDermott and J. Piatigorsky. 1995. cDNA encoding a chicken protein with homology to hnRNP type A/B. Biophys. Biochim. Acta. 1261:290-292.

19.       Sax, C.M., A. Cvekl, M. Kantorow, R. Gopal-Srivastava, J.G. Ilagan, N.P. Ambulos, Jr. and J. Piatigorsky. 1995. Lens-specific activity of the mouse aA-crystallin promoter in the absence of a TATA box: Functional and protein binding analysis of the mouseaA-crystallin PE1 region. Nucleic Acids Res. 23:442-451.

20.      Cvekl, A., C.M. Sax, X. Li, J.B. McDermott and J.Piatigorsky. 1995. Pax-6 and lens-specific transcription of the chickend1-crystallin gene. Proc. Natl. Acad. Sci. USA 90:4681-4685.

21.       Richardson, J., A. Cvekl and G. Wistow. 1995. Pax-6 is essential for lens-specific expression of z-crystallin. Proc. Natl. Acad. Sci. USA 90:4676-4680.

22.       Duvall, J.F., F. Kashanchi, A. Cvekl, M.F. Radonovich and J.N. Brady. 1995. Transactivation of the HTLV-I Tax1-responsive 21 bp repeats requires holo-TFIID and TFIIA. J. Virol. 69:5077-5086.

23.       Chambers, C., A. Cvekl, C.M. Sax and P. Russell. 1995. Sequence, initial functional analysis and protein-DNA binding sites of the mouse bB2-crystallin-encoding gene. Gene (Amst.) 166: 287-292.

24.       McDermott, J.B., A. Cvekl and J. Piatigorsky. 1996. Lens-specific  expression of the chicken bA3/A1-crystallin promoter fragment in transgenic mice. Biochem. Biophys. Res. Commun. 221:559-564.

25.       Gopal-Srivastava, R., A. Cvekl and J. Piatigorsky. 1996. Regulation of the mouse aB-crystallin/small heat shock protein gene in lens: involvement of Pax-6. J. Biol. Chem. 271:23029-23036.

26.       Sax, C.M., A. Cvekl and J. Piatigorsky. 1997. Transcriptional regulation of the mouse aA-crystallin gene: binding of USF to -7/ 5 region. Gene (Amst.) 185:209-216.

27.       McDermott, J.B., A. Cvekl and J. Piatigorsky. 1997. A complex enhancer of the chicken bA3/A1-crystallin gene depends on an AP-1/CRE element for activity. Invest. Ophthalmol. Vis. Sci. 38:951-959.

28.       Li, X., A. Cvekl, S. Bassnett, and J. Piatigorsky. 1997. Lens-preferred activity of chicken d1-andd2-crystallin enhancers in transgenic mice and evidence for retinoic acid-responsive regulation of thed1-crystallin gene. Dev. Genet. 20:258-266. 

29.       Gopal-Srivastava, A. Cvekl and J. Piatigorsky. 1998. Involvement of retinoic acid/retinoid receptors  in the regulation of murine aB-crystallin/small heat shock protein gene expression in the lens. J. Biol. Chem. 273:17954-17961.

30.       Duncan, M.K., J.I. Haynes, A. Cvekl, and J. Piatigorsky. 1998. Dual roles for Pax-6: a transcriptional repressor of lens fiber-cell specific b-crystallin genes. Mol. Cell. Biol. 18:5579-5586.

31.      Cvekl, A., F. Kashanchi, J.N. Brady and J. Piatigorsky. 1999. Pax-6 interactions with TATA-box binding protein and retinoblastoma protein.  Invest. Ophthalmol. Vis. Sci. 40:1343-1350.

32.       Ilagan, J.G., A. Cvekl, M. Kantorow, J. Piatigorsky, and C.M. Sax. 1999. Regulation of aA-crystallin gene expression: lens-specificity achieved through the differential placement of similar transcriptional control elements in mouse and chicken. J. Biol. Chem. 274:19973-19978.

33.       Kozmik, Z., J. Paces, J. Kralova, P. Pfeffer, V. Paces, A. Kalousova & A. Cvekl. 1999. Molecular cloning and expression of the human and mouse homologues of the Drosophiladachshund gene. Dev. Genes Evol. 209:537-545.

34.       Kozmik, Z., and A. Cvekl. 1999. Localization of the human homologue of the Drosophila dachshund (DACH) gene to chromosome 13q21. Genomics 59:110-111.

35.       Duncan, M.K., A. Cvekl, X. Li, and J. Piatigorsky. 2000. Truncated forms of Pax-6 can disrupt lens morphology in transgenic mice. Invest. Ophthalmol. Vis. Sci. 41:464-473.

36.       Duncan, M.K., Z. Kozmik, K. Cveklova, J. Piatigorsky, and A. Cvekl.  2000. Overexpression of PAX6(5a) in lens fiber cells results in upregulation ofa5b1 integrin expression.  J. Cell Sci. 113:3173-3185.

37.       Kirstein, L., A. Cvekl, B.K. Chauhan, and E.R. Tamm. 2000.Regulation of human Myocillin/TIGR gene transcription in trabecular meshwork cells and astrocytes: role of Upstream Stimulator Factor. Genes Cells 5: 661-676.

38.       Zhang, W., K. Cveklova, B. Oppermann, M. Kantorow, and A. Cvekl. 2001. Quantitation of PAX6 and PAX6(5a) transcript levels in adult human lens, cornea, and monkey retina. Mol. Vis. 7:1-6.

39.       Chauhan, B.K., N.A. Reed, W. Zhang, M.K. Duncan, M. Kilimann, and A. Cvekl. 2002. Identification of genes downstream of Pax6 in the mouse lens using cDNA microarrays. J. Biol. Chem. 277:11539-11548.

40.       Chauhan, B.K., W. Zhang, K. Cveklova, M. Kantorow, and A. Cvekl. 2002. Identification of differentially expressed genes in mouse Pax6 heterozygous lenses. Invest. Ophthalmol. Vis. Sci. 43:1884-1890.

41.       Sheets, N., B.K. Chauhan, E. Wawrousek, F.J. Hejmancik, A. Cvekl, and M. Kantorow. 2002. Cataract- and lens-specific up-regulation of ARK receptor tyrosine kinase in Emory mouse cataract. Invest. Ophthalmol. Vis. Sci. 43:1870-1875.

42.       Kim, S.-S., R. Zhang, S.E. Braunstein, A. Joachimiak, A. Cvekl, and R.S. Hegde. 2002. Structure of the retinal determination protein Dachshund reveals a DNA binding motif. Structure 10: 787-795.

43.       Baulmann, D.C., A. Ohlmann, C. Flugel-Koch, S. Goswami, A. Cvekl, and E.R. Tamm. 2002. Pax6 heterozygous eyes show defects in chamber differentiation that are associated with a wide spectrum of other anterior eye segment abnormalities. Mech. Develop. 118:3-17.

44.       Chauhan, B.K., N.A. Reed, Y. Yang, L. Cermak, L. Reneker, M.K. Duncan, M. and A. Cvekl. 2002. A comparative cDNA microarray analysis reveals a spectrum of genes regulated by Pax6 in mouse lens. Genes Cells 7:1267-1283.

45.       Goswami, S., N. L. Sheetz, J. Zavadil, B.K. Chauhan, E.P. Bottinger, V.N. Reddy, M. Kantorow, and A. Cvekl. 2003. The spectrum and range of oxidative stress responses of human lens epithelial cells to H2O2 insult. Invest. Ophthalmol. Vis. Sci. 44:2084-2093.

46.       Wu, K., Y. Yang, C. Wang, M.A. Davoli, M. D'Amico, A. Li, K. Cveklova, Z. Kozmik, M.P. Lisanti, R.G. Russel, A. Cvekl, and R.G. Pestell. 2003. DACH1 inhibits TGF-b signaling through binding Smad4. J. Biol. Chem. 278:51673-51684.

47.       Chauhan, B.K., Y. Yang, K. Cveklova, and A. Cvekl. 2004. Functional properties of natural human PAX6 and PAX6(5a) mutants. Invest. Ophthalmol. Vis. Sci. 45: 385-392.

48.       Chauhan, B.K., Y. Yang, K. Cveklova, and A. Cvekl. 2004. Functional interactions between alternatively spliced forms of Pax6 in crystallin gene regulation and in haploinsufficiency. Nucleic Acids Res. 32:1696-1709.

49.       Zaniolo, K., S. Leclerc, A. Cvekl, R. Bazin, K. Larouche, and S.L. Guerin. 2004. Expression of thea4 integrin subunit gene promoter is modulated by the transcription factor Pax-6 in corneal epithelial cells. Invest. Ophthalmol. Vis. Sci. 45: 1692-1704.

50.       Cvekl, A., B.K. Chauhan, Y. Yang, and K. Cveklova. 2004. Regulation of gene expression by Pax6 in ocular cells: A case of tissue-preferred expression of crystallins in lens. Int. J. Dev. Biol. 48:829-844.

51.       Yang, Y., B.K. Chauhan, K. Cveklova, and A. Cvekl. 2004. Transcriptional regulation of mouseaB- andgF-crystallin genes in lens: Dual promoter-specific functional interactions between Pax6 and large Maf transcription factors. J. Mol. Biol. 344: 351-368.

52.       Cvekl, A., Jr., J. Zavadil, B.K. Birshtein, M. Grotzer and A. Cvekl. 2004. Analysis of transcripts from 17p13.3 in medulloblastoma suggests ROX/MNT as a potential tumour suppressor gene. Eur. J. Cancer 40: 2525-2532.

53.       Ohlmann A., M. Scholz, A. Goldwich, B.K. Chauhan, K. Hudl, A.V. Ohlmann, E. Zrenner, W. Berger, A. Cvekl, M.W. Seelinger and E.R. Tamm. 2005.Ectopic norrin induces growth of ocular capillaries and restores normal retinal angiogenesis in Norrie disease mutant mice. J. Neurosci. 25:1701-1710.

54.       Yang, Y. and A. Cvekl. 2005. Tissue-specific regulation of the mouseaA-crystallin gene in lens via recruitment of Pax6 and c-Maf to its promoter. J. Mol. Biol. 351:453-469.

55.       Castellini, M., L.V. Wolf, B.K. Chauhan, M.W. Kilimann, A. Cvekl and M.K. Duncan. 2005. Palm is expressed in both developing and adult mouse lens and retina. BMC Ophthalmology 5:14.

56.       Wu, K., A. Li, C. Wang, M. Rao, M. Liu, V. Dailey, Y. Yang,  M.P. Lisanti, D. Di Vizio, C. Wang, M.P. Lisanti, G. Sauter, R.G. Russell, A. Cvekl, and R.G. Pestell. 2006. DACH1 is a cell fate determination factor that inhibits cyclin D1 and breast tumor growth. Mol. Cell. Biol. 26:7116-7129.

57.       Yang Y., T. Stopka, N. Golestaneh, Y. Wang, K. Wu, A. Li, B.K. Chauhan, C.Y. Gao, K. Cveklova, M.K. Duncan, R.G. Pestell, A.B. Chepelinsky, A.I. Skoultchi and A. Cvekl. 2006. Regulation ofaA-crystallin via Pax6, c-Maf, CREB, and a broad domain of lens-specific chromatin. EMBO J. 25:2107-2118.

58.       Wu, K., M. Liu, A. Li, H. Donninger, M. Rao, X. Jiao, M.P. Lisanti, A. Cvekl, M. Birrer, and R.G. Pestell. 2007. Cell fate determination factor DACH1 inhibits c-Jun-induced contact-independent growth. Mol. Biol. Cell 18:755-767.

59.       Yang, Y., L.V. Wolf, and A. Cvekl. 2007 Distinct embryonic expression and localization of CBP and p300 histone acetyltransferases at the mouseaA-crystallin locus in lens. J. Mol. Biol. 369:917-926.

60.       Pirity, M.K., W.-L. Wang, L.V. Wolf, E.R. Tamm, N. Schreiber-Agus and A. Cvekl. 2007. Rybp, a polycomb complex-associated protein, is required for mouse eye development. BMC Dev. Biol. 7:39.

61.       Pontoriero, G.F., P. Deschamps, R. Ashery-Padan, R. Wong, Y. Yang, J. Zavadil, A. Cvekl, S. Sullivan, T. Williams and J.A. West-Mays. 2008. Cell autonomous roles  for AP-2ain lens vesicle separation and maintenance of the lens epithelial cell phenotype. Dev. Dynam. 237: in press.

B.        Books, Chapters in Books, and Review Articles.           

  1. Cvekl, A., K. Horska and K. Sebesta. 1986. Transcription in Escherichia coli. Chemicke listy 80:954-976 (in Czech).
  2. Cvekl, A., K. Horska and K. Sebesta. 1989. Initiation of transcription in prokaryotes. Chemicke listy 83:29-56 (in Czech).
  3. Cvekl, A. 1990. Transcription in eukaryotic cell. In New Trends in Molecular Genetics II (V. Benes, ed.). Institute of Molecular Genetics Prague, pp. 9-18 (in Czech).
  4. Cvekl, A. 1991. Transcription in eukaryotes. Vesmir 70:73-75.
  5. Cvekl, A.1991. Protein TFIID: A basic transcription factor in eukaryotes. Chemicke listy 85:398-406 (in Czech).
  6. Cvekl, A. and Piatigorsky, J. 1996. Lens development and crystallin gene expression:  many roles for Pax-6. BioEssays 18:621-630. (Cover article).
  7. Duncan, M.K.*, Cvekl, A.*, Kantorow, M., and Piatigorsky, J. 2004. Lens crystallins. In Ocular lens development (M.L. Robinson and F.J. Lovicu, Eds.), Cambridge University Press, pp. 119 - 150. (* Equal contributions).
  8. Cvekl, A. and E.R. Tamm. 2004. Anterior eye development and ocular mesenchyme: new insights from studies of Pax6 and other DNA-binding transcription factors. BioEssays 26:374-386. (Cover article).
  9. Yang, Y. and A. Cvekl. 2007. Large Maf transcription factors: cousins of AP-1 proteins and important regulators of cellular differentiation. Einstein J. Biol. Med. 23: 2-11.
  10. Cvekl, A. and M.K. Duncan. 2007. Genetic and epigenetic mechanisms of gene regulation during lens development. Prog. Retinal Eye Res. 26:555-597.

 

          * joint authorship 

 

 

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