Ullmann 614
Overview
The Imaging and Cell Structure Core of the Liver Research Center operates in conjunction with the Analytical Imaging Facility (AIF) to provide a state-of-the-art resource for modern fluorescence and electron microscopy for Liver Research Center (LRC) Investigators.
The Core facilitates use of imaging techniques in liver research by supporting and assisting with use of specialized instrumentation, including laser scanning confocal microscopy, deconvolution microscopy, multi-photon microscopy, transmission electron microscopy (TEM), scanning electron microscopy (SEM), cryo-electron microscopy (cryo-EM) and correlative light and electron microscopy (CLEM). In addition, the Core provides expertise in and assistance with specialized imaging techniques such as vesicle tracking, volumetric measurements, ultrastructural sample preparation, super resolution fluorescence microscopy (STORM/SIM), single-molecule Fluorescence in situ Hybridization (smFISH), Fluorescence Recovery After Photobleaching (FRAP), Fluorescence Resonance Energy Transfer (FRET) and Total Internal Reflection Microscopy (TIRF).
The Core provides technical support for assisted or independent use of the imaging technology and helps in the design of experiments, data acquisition and analysis related with relevant studies on hepatocytes and liver tissue.
An extensive selection of fluorescent protein vectors, fluorescent antibodies and probes, cell dye labeling technologies, and advice in the design of light and electron microscopy experiments is available.
Histopathology needs of LRC members are served by the Histology and Comparative Pathology Facility for animal tissues; and, a consulting group of pathologists led by Dr. Kathryn Tanaka at Montefiore Hospital for human tissue samples.
Services and Technology
A. Light Microscopy
A.1) Widefield Epifluorescence Microscopes:
- Zeiss AxioObserver with Shuttle and Find
A.2) Laser Confocal Microscopes:
- Leica AOBS SP5 Laser Scanning Confocal Microscope
- Leica AOBS SP8 Confocal Microscope
- Zeiss LSM5 Live/DuoScan Confocal Microscope
- PerkinElmer UltraVIEW ERS Spinning Disk Confocal Microscope
A.3) Multiphoton Microscopes:
- Olympus Multiphoton PV1000-MPE
A.4) Scanners:
- PerkinElmer P250 High Capacity Slide Scanner (Brightfield and Fluorescence)
A.5) Super Resolution Microscopes:
- Nikon Super Resolution Microscope (TIRF, 2D-STORM, 3D-STORM, 3D-SIM)
A.6) Other Light Microscopic Techniques:
- Volume Rendering and 3D Quantitation
- D.I.C. (Nomarski), Darkfield, Phase Contrast, IRM, and Epifluorescence
- Deconvolution
- Particle and Cell Tracking
- Microinjection Förster Resonance Energy Transfer (FRET)
- Single-molecule Fluorescence in situ hybridization (smFISH)
- FRAP, FLIP, Photoactivation, iFRAP, and other photo manipulation techniques
- Total Internal Reflection Fluorescence Microscopy (TIRF)
B. Electron Microscopy
B.1) Transmission Electron Microscopes:
- JEOL 1400Plus Transmission Electron Microscope
- JEOL 1200EX Transmission Electron Microscope
- FEI Tecnai 20 Cryo Electron Microscope
B.2) Scanning Electron Microscope:
- Zeiss Supra 40 Field Emission Scanning Electron Microscope
B.3) Specimen Preparation for Electron Microscopy
C. Image Analysis
C.1) Image Digitalization and Quantification:
- Image Analysis Software Support
- Time Lapse Motion Analysis
- Co-localization
- Diffusion analysis
- Full service sample preparation for TEM and SEM
D. Animal Histology Services
- Consultation and training on technical approaches, dissection, and tissue preparation
- Histopathology interpretation
- Optimization of histochemistry and immunohistochemistry
- General immunohistochemistry
- Histopathological analysis of embryonic tissues
E. Human Liver Histopathology
F. Fluorescent Protein Resource Center
- Fluorescent protein plasmids library, expression vectors, fluorescent dye-labeled secondary antibodies, fluorescent organelle markers
For additional information, please contact Carolina Eliscovich, Ph.D.