Macromolecular Therapeutics Development

The Macromolecular Therapeutics Development Facility (MTDF) provides expertise for directed development and optimization of protein-based therapeutics and supports high-throughput automated development, analysis and production of protein reagents using a Bioemk FxP  liquid handling workstation for HTP molecular biology and expression testing, a Perkin-Elmer Cell::Explorer robot for very efficient HTP tissue culture based application and a custom microarray printer. This automation is designed to provide Einstein researchers with the added leverage of robotics equipment and trained personnel to accelerate and scale up their projects, thereby obviating the need for individual investigators to set up and staff their own facilities for protein production.

Services

Access the Services

  • General cloning into a range of protein expression vectors (single through 96 well)
  • Expression profiling – E. coli, insect, mammalian
  • Large-scale and high-throughput growth of cells or bacteria expressing recombinant proteins
  • Classical and automated purification of recombinant proteins from culture supernatant
  • Custom DNA and cell microarrays
  • Generation of hybridomas making mouse and rat monoclonal antibodies
  • Generation of isotype switched monoclonal antibodies
Related Shared Facilities
Related Shared Facilities

Structural NMR

X-Ray Crystallography

Center Affiliations
Center Affiliations

Montefiore Einstein Comprehensive Cancer Center

Selected Publications

Biomed Central Microbiology,2008 Sep 23;8:159 Clinical and Vaccine Immunology,2007 Sep;14(9):1094-101 Journal of Immunological Methods,2006 Oct; 316(1-2):59-66

Location and Contacts

Macromolecular Therapeutics Development Price Center/Block Research Pavillion 313