Gene Targeting and Transgenic Facility The Gene Targeting and Transgenic Facility is a centralized facility for the production of transgenic and gene-targeted mice. The facility’s services allow researchers to modify the mouse genome through methods of conventional transgenesis. In addition, the facility is integrated with the Gene Modification facility to generate gene-targeted mice using CRISPR/Cas9 technology. It also offers services for BAC transgenesis and the generation of chimeric mice by injection of embryonic stem cells into blastocysts. Finally, the facility offers a wide variety of reproductive services including in vitro fertilization and mouse line re-derivation, sperm and embryo cryopreservation, and storage of cryopreserved sperm and embryos. Services Access the Services COMING SOON:GENE MODIFICATION SERVICES GENE TARGETING SERVICES: Consultation on targeting vector construction, ES cell culture, ES cell screening methods, mouse genotyping and maintenace of gene targeted mice. Chimera Production: Blastocyst Injection 6-8 cell embryro injection for high yield chimera production ES Cell Culture Service: Construct electroporation Isolation of targeted clones Preparation for blastocyst injection Related Shared Facilities Gene ModificationTransgenic Mouse Center Affiliations Montefiore Einstein Cancer Center Selected Publications no-publications Location and Contacts Gene Targeting and Transgenic Facility Price Center/Block Research Pavillion B110 Winfried Edelmann, Ph.D.Scientific Director718-678-1086winfried.edelmann@einsteinmed.eduKen Chen, M.D.Operations Director718-678-1106ken.chen@einsteinmed.edu