Gene Targeting and Transgenic Facility

The Gene Targeting and Transgenic Facility is a centralized facility for the production of transgenic and gene-targeted mice. The facility’s services allow researchers to modify the mouse genome through methods of conventional transgenesis. In addition, the facility is integrated with the Gene Modification facility to generate gene-targeted mice using CRISPR/Cas9 technology. It also offers services for BAC transgenesis and the generation of chimeric mice by injection of embryonic stem cells into blastocysts. Finally, the facility offers a wide variety of reproductive services including in vitro fertilization and mouse line re-derivation, sperm and embryo cryopreservation, and storage of cryopreserved sperm and embryos.

  • Consultation on targeting vector construction, ES cell culture, ES cell screening methods, mouse genotyping and maintenace of gene targeted mice.
  • Chimera Production:
  • Blastocyst Injection
  • 6-8 cell embryro injection for high yield chimera production
  • ES Cell Culture Service:
  • Construct electroporation
  • Isolation of targeted clones
  • Preparation for blastocyst injection

Related Shared Facilities
Related Shared Facilities

Gene Modification

Transgenic Mouse

Center Affiliations
Center Affiliations

Montefiore Einstein Cancer Center

Selected Publications


Location and Contacts

Gene Targeting and Transgenic Facility Price Center/Block Research Pavillion B110
  • Winfried Edelmann, Ph.D.
    Scientific Director

  • Ken Chen, M.D.
    Operations Director