Hepatic Animal Models, Stem Cells and Cell Therapy This Core breeds unique animals of interest, reproduces animal models, provides training in procedures and consultation in experimental design. New technologies are developed for animal and human stem cell and cell therapy studies. Primary animal or human liver cells of consistently high quality are isolated and distributed. Liver cells generated from pluripotent stem cells with highly efficient differentiation protocols can also be provided. Animal use requires ACUC approval for the investigator’s project. Disease models include analbuminemic rat, Gunn rat, LEC rat and Atp7b-/- mice (Copper toxicosis), and hemophilia A mice, along with additional rat and mouse models for cell transplantation research. The Core offers training in specialized liver procedures, e.g., partial hepatectomy, bile duct ligation or cannulation, portal vein cannulation, liver perfusion, induction of liver injury or fibrosis, etc. The Core can provide specialized cell culture media and additives for hepatocytes. Services Access the Services The Core breeds unique animals of interest, reproduces animal models, provides training in procedures, and consultation in experimental design. Animal use requires ACUC approval. Animals bred by the Core: Inbred Gunn rats (model for Crigler-Najjar syndrome) and congeneic Wistar-RHA rats Inbred Long-Evans Cinnamon (LEC) rats (model for Wilson disease) and healthy LEA rats Dipeptidyl peptidase IV mutant (DPPIV) F344 rats and DPPIV-/- knockout mice Hemophilia A knockout mice Induced animal models: Liver injury with D-GalN, CCl4, allyl alcohol, thiacetamide, azopxymethane, concavalin A, etc. Induction of cirrhosis in rats with chronic CCl4 administration, bile duct ligation Animal procedures: Partial hepatectomy (68%, 35%) in rats, mice, rabbits, etc. Intrasplenic, intraportal, subcutaneous injections, gallbladder or bile duct cannulation Liver perfusion, tissue fixation in situ, etc. Related Shared Facilities no-related-facilities Center Affiliations Marion Bessin Liver Research Center Selected Publications no-publications Location and Contacts Hepatic Animal Models, Stem Cells and Cell Therapy Ullmann 505, 511, 610 Sanjeev Gupta, M.D.Scientific Director718-430-3309sanjeev.gupta@einsteinmed.edu