Macromolecular Therapeutics Development The Macromolecular Therapeutics Development Facility (MTDF) provides expertise for directed development and optimization of protein-based therapeutics and supports high-throughput automated development, analysis and production of protein reagents using a Bioemk FxP liquid handling workstation for HTP molecular biology and expression testing, a Perkin-Elmer Cell::Explorer robot for very efficient HTP tissue culture based application and a custom microarray printer. This automation is designed to provide Einstein researchers with the added leverage of robotics equipment and trained personnel to accelerate and scale up their projects, thereby obviating the need for individual investigators to set up and staff their own facilities for protein production. Services Access the Services *Contact Ms. Susan Buhl for Monoclonal Antibodies CLONING: State of the art Robotics platforms for 96 well High Throughput Vector Backbone Optimization (pET vector screen) Purification Tag Optimization (including Linker Length) 96 well high throughput mass mutagenesis. Potential uses include stability profiling and solubility enhancement. EXPRESSION: E.Coli Shake Flask (48 Liter Capacity) Single Cell (2 L) Fermentation High Throughout 96 well (65ml) Fermentation Baculovirus (SF9, S2) Shake Flasks (10 L Capacity) Mammalian (HEK293T) Roller Bottle (48 1/2 liter capacity) PURIFICATION: Column Purification, all types (Affinity, Size Exclusion, etc) AKTA Express systems for hands off automated purification of 8 AKTA Purifier for single step purifications MONOCLONAL ANTIBODY GENERATION AND PRODUCTION: Generation of hybridomas making mouse or rat monoclonal antibodies Immunization of animals Fusions to generate hybridomas Assay of antibody responses and identification of mice for fusions Screening of fusions for positive hybridomas Subcloning of positive hybridomas Freezing of useful hybridomas Maintain a backup of frozen stocks Generation of class switch variants of existing monoclonal antibodies Production of high concentrations of monoclonal antibodies CONSULTATION ON: Cloning,expression and purification of protein Strategies to obtain useful monoclonal antibodies Letters in support of grant applications Related Shared Facilities Structural NMR X-Ray Crystallography Center Affiliations Montefiore Einstein Cancer Center Selected Publications Biomed Central Microbiology,2008 Sep 23;8:159 Clinical and Vaccine Immunology,2007 Sep;14(9):1094-101 Journal of Immunological Methods,2006 Oct; 316(1-2):59-66 Location and Contacts Macromolecular Therapeutics Development Price Center/Block Research Pavillion 313 Steven C. Almo, Ph.D.Scientific Director718-430-2746steve.almo@einsteinmed.orgScott Garforth, Ph.D.Operations Director718.678.1146scott.garforth@einsteinmed.orgMatthew D. Scharff, M.D.Co-Scientific Director (Monoclonal Antibodies)718-430-3527matthew.scharff@einsteinmed.orgSusan BuhlAssociate Director (Monoclonal Antibodies)718-430-2170susan.buhl@einsteinmed.org