X-Ray Crystallography The X-Ray Crystallography Facility provides the expertise needed to obtain high-resolution structures by X-ray diffraction, including crystallization, primary phasing, structure refinement and data interpretation. To rapidly accomplish these tasks, the facility is equipped with state-of-the-art robotics for screening sub-microliter volumes of protein and RT-PCR based stability tests to enhance crystallization efforts. All data are collected using synchrotron radiation using our rapid and sustained access to beamlines at Brookhaven National Labs. These structural technologies are complemented by cryo-electron microscopy, electron paramagnetic resonance, a wide range of vibrational spectroscopies, novel macromolecular footprinting approaches and computational biology. A full list of services is available on the facility website. Services Access the Services PROTEIN PRODUCTION: Consultation on the expression, purification and handling of materials for crystallization and subsequent structure determination CRYSTALLIZATION: Provision of all required expertise and instrumentation for initial screening and optimization of protein crystals Automation is available for crystallization in 96-well format, including a TECAN FREEDOM-2000 crystallization robot and a PHENIX crystallization robot DATA COLLECTION AND STRUCTURE DETERMINATION: Expertise for all aspects of data collection, structure determination and refinement Equipped in-house with an RU-300 X-ray generator coupled to an R-Axis image plate detector with focusing optics and low temperature capabilities for cryogenic data collection Extensive access to a number of synchrotron X-ray sources at Brookhaven National Laboratory and the Advanced Photon Source STRUCTURE INTERPRETATION AND PROJECT DESIGN: Extensive expertise in the interpretation of structural results and can be of considerable assistance in the design and interpretation of experiments based on macromolecular structure Related Shared Facilities Structural NMR Center Affiliations Montefiore Einstein Cancer Center Selected Publications Biochemistry, 2008 May 6;47(18):5111-26 Nature Structural and Molecular Biology, 2008 Jan;15(1):103-5 Proceedings of the Natl Academy of Sciences USA, 2008 Jan 15;105(2):635-40 Proceedings of the Natl Academy of Sciences USA,2008 Jul 29;105(30):10483-8 Location and Contacts X-Ray Crystallography Ullmann 409 Steven C. Almo, Ph.D.Scientific Director718-430-2746steve.almo@einsteinmed.eduJeffrey B. Bonanno, Ph.D.Associate Director718.430.2743jeffrey.bonanno@einsteinmed.edu