The
staff of the AIF offers full service sample preparation for many standard and
state of the art EM techniques. These include:
- Embedding utilizing either epoxy or acrylic resins at ambient or low temperatures
- Thin Sectioning with a Leica EMUC7, Leica UCT or Reichert Ultracut E Ultramicrotome
- Negative Staining of viruses and bacteria
- Freeze Fracture using a Cressington CFE-50 Freeze Etch Unit
- Immunogold Labeling following pre or post embedding protocols
- Cryoultramicrotomy utilizing a Leica UCT cryoultramicrotome
- Tokyasu immunogold labeling of ultrathin cryo sections
- Critical Point Drying with a Tousimis Samdri 790 Critical Point Dryer
- Sputter Coating using a EMS 150TES coating unit
- Slam Freeze Cryofixation using a Life Cell CF100 Slam Freezer
- High Resolution Rotary Shadowing in a Cressington CFE-50
- High Pressure Freezing using a Bal-Tec HPM-010 High Pressure Freezing Machine
- Freeze Substitution and Low Temperature Embedding in a RMC FS-7500 Freeze Substitution
Unit
- Plunge Freezing of macromolecules for cryoEM using a FEI Vitrobot
Electron Microscopes
Electron Microscopy Preparation